FAM azide,6-isomer|1386385-76-7
 
FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.
Pure 6-isomer.
Can replace Alexa Fluor 488, DyLight 488.
 
 
FAM azide,6-isomer|1386385-76-7
 
General properties：
Appearance:
yellowish crystals
Molecular weight:
458.42
CAS number:
1386385-76-7
Molecular formula:
C24H18N4O6
Solubility:
soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control:
NMR 1H, HPLC-MS (95%)
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
 
Spectral properties：
Excitation maximum, nm:
494
ε, L⋅mol−1⋅cm−1:
75000
Emission maximum, nm:
520
Fluorescence quantum yield:
0.9
CF260:
0.20
CF280:
0.17
       常用荧光染料的激发波长及发射波长
荧光染料
Ex（nm）
Em（nm）
FITC
490
525
TRITC
547
572
Cy2
489
506
Cy3
555
570
Cy5
646
620
Cy5.5
675
694
Cy7
750
773
Calcein
494
517
Rhodamine 110
496
520
Rhodamine B
555
580
5-Carboxy Fluorescein
492
518
5-FAM, SE
492
520
6-TAMRA SE
543
575
6-Carboxy-X-rhodamine
568
590
Alexa TM 488
490
520
BODIPY
530
550
Alexa TM 546
555
570
Pyronin Y
550
580
Thiadicarbocyanine
651
670
Alexa TM 594
590
615
6-Aminofluorescein
490
520
Dansyl chloride
337
492
Rhodamine 123
507
529
4-Methylumbelliferone
385
502
5-carboxy-2,7-dichlorofluorescein
504
529
5-Carboxynapthofluorescein
512
568
5-Carboxytetramethylrhodamine (5-TAMRA)
542
568
5-FAM (5-Carboxyfluorescein)
492
518
5-ROX (carboxy-X-rhodamine)
567
591
5-TAMRA (5-Carboxytetramethylrhodamine)
548
552
Acriflavin
436
520
Alexa Fluor 350™
346
442
Alexa Fluor 430™
431
540
Alexa Fluor 488™
492
520
Alexa Fluor 532™
531
553
Alexa Fluor 568™
577
603
Alexa Fluor 633™
632
650
Alexa Fluor 680™
679
702
AMCA（Aminomethylcoumarin）
345
425
Aminocoumarin
346
442
Anthrocyl stearate
381
446
APC-Cy7
655
755
Atabrine
436
490
Aurophosphine
490
515
Bisbenzamide
360
461
Bodipy 492/515
490
515
Bodipy 500/510
500
510
Bodipy 530/550
528
547
Bodipy 576/589
579
590
Catecholamine
410
470
Coumarin Phalloidin
387
470
Dansyl Amine
337
517
Dansyl Chloride
372
518
Hydroxycoumarin
325
386
NBD
466
539
PerCP
488
675
Nile Red
556
590
Noradrenaline
340
490
Thiolyte
385
488
Merocyanin
555
578
BODIPY TMR
542
568